Dartmouth Electron Microscope Facility Date: Fri 04-Nov-2011 Time: 14:40
01_RootNodules80936 5.0KX.jpg Instrument: JEOL TEM 1010
Microscopist: Louisa Howard

Transmission electron microscope image of a cross section through a soybean(Glycine max.Essex) root nodule. The bacteria, Bradyrhyzobium japonicum, infects the roots and establishes a nitrogen fixing symbiosis. This image shows a cell and an infection thread. In this image, you can also see a cell in process of dividing.
For more information, see the articles:
Benson HP, Boncompagni E, Guerinot ML (2005) "An iron uptake operon required for proper nodule development in the Bradyrhizobium japonicum-soybean symbiosis" Mol Plant Microbe Interact. 2005 Sep;18(9):950-9.
Roth, E. and G. Stacey (1989) "Bacterium release into host cells of nitrogen-fixing soybean nodules"  J.C.B. Vol. 49 pp.13-23 1989
02_RootNodules80938 50KX.jpg Instrument: JEOL TEM 1010
Microscopist: Louisa Howard
Transmission electron microscope image of a cross section through a soybean(Glycine max.Essex) root nodule. The bacteria, Bradyrhyzobium japonicum, infects the roots and establishes a nitrogen fixing symbiosis. This high magnification image shows part of a cell with single bacteriods within their symbiosomes. In this image, you can also see endoplasmic reticulum and the cell wall.
For more information, see the articles:
Benson HP, Boncompagni E, Guerinot ML (2005) "An iron uptake operon required for proper nodule development in the Bradyrhizobium japonicum-soybean symbiosis" Mol Plant Microbe Interact. 2005 Sep;18(9):950-9.
Roth, E. and G. Stacey (1989) "Bacterium release into host cells of nitrogen-fixing soybean nodules"  J.C.B. Vol. 49 pp.13-23 1989
03_RootNodules80932 6.6KX.jpg Instrument: JEOL TEM 1010
Microscopist: Louisa Howard
Transmission electron microscope image of a cross section through a soybean(Glycine max.Essex) root nodule. The bacteria, Bradyrhyzobium japonicum, infects the roots and establishes a nitrogen fixing symbiosis. This image shows a cell with single bacteriods within their symbiosomes.
For more information, see the articles:
Benson HP, Boncompagni E, Guerinot ML (2005) "An iron uptake operon required for proper nodule development in the Bradyrhizobium japonicum-soybean symbiosis" Mol Plant Microbe Interact. 2005 Sep;18(9):950-9.
Roth, E. and G. Stacey (1989) "Bacterium release into host cells of nitrogen-fixing soybean nodules"  J.C.B. Vol. 49 pp.13-23 1989
04_RootNodules80937 33KX.jpg Instrument: JEOL TEM 1010
Microscopist: Louisa Howard
Transmission electron microscope image of a cross section through a soybean(Glycine max.Essex) root nodule. The bacteria, Bradyrhyzobium japonicum, infects the roots and establishes a nitrogen fixing symbiosis. This high magnification image shows part of a cell with single bacteriods within their symbiosomes. In this image, you can also see endoplasmic reticulum and cell wall.
For more information, see the articles:
Benson HP, Boncompagni E, Guerinot ML (2005) "An iron uptake operon required for proper nodule development in the Bradyrhizobium japonicum-soybean symbiosis" Mol Plant Microbe Interact. 2005 Sep;18(9):950-9.
Roth, E. and G. Stacey (1989) "Bacterium release into host cells of nitrogen-fixing soybean nodules"  J.C.B. Vol. 49 pp.13-23 1989
05_RootNodules80928 3.3KX.jpg Instrument: JEOL TEM 1010
Microscopist: Louisa Howard
Transmission electron microscope image of a cross section through a soybean(Glycine max.Essex) root nodule. The bacteria, Bradyrhyzobium japonicum, infects the roots and establishes a nitrogen fixing symbiosis. This image shows cells, with bacteriods within their symbiosomes, and infection threads
For more information, see the articles:
Benson HP, Boncompagni E, Guerinot ML (2005) "An iron uptake operon required for proper nodule development in the Bradyrhizobium japonicum-soybean symbiosis" Mol Plant Microbe Interact. 2005 Sep;18(9):950-9.
Roth, E. and G. Stacey (1989) "Bacterium release into host cells of nitrogen-fixing soybean nodules"  J.C.B. Vol. 49 pp.13-23 1989
06 2b-coleusleaf-4.jpg Instrument: JEOL TEM 1010
Microscopist: Louisa Howard

Lamiales
Lamiaceae (mint family)
Coleus blumei

Transmission electron microscope image of a chloroplast. There are starch granules present, along with the grana (stacks of disc-shaped thylakoids).


07 44568_1Cplate-1.jpg Instrument: JEOL TEM 1010
Microscopist: Louisa Howard

Asterales
Asteraceae (daisy family)
Latuca sativa
Transmission electron microscope image of a thin section cut from Latuca sativa root tip specimen. Image shows formation of the cell plate, during cell division. A layer of vesicles in the midplane between the the two sets of daughter chromosomes forms an incomplete cell plate. In its further growth, the cell plate will traverse the cytoplasm and reach the cell wall.
08 55097_2Cplate-1.jpg Instrument: JEOL TEM 1010
Microscopist: Louisa Howard

Lamiales
Lamiaceae (mint family)
Coleus Blumei

Transmission electron microscope image of a thin section cut from Coleus Blumei shoot apex specimen. Image shows formation of the cell plate, during cell division. A layer of vesicles, formed in the midplane between the the two sets of daughter chromosomes, is called the cell plate. In this image, the cell plate has almost traversed the entire cytoplasm and reached the cell wall.
09 55108_Cplate_extra-1.jpg Instrument: JEOL TEM 1010
Microscopist: Louisa Howard

Lamiales
Lamiaceae (mint family)
Coleus Blumei

High maginfication of "2CPLate" image, showing a portion of the cell plate. At this stage of cell division, the cell plate is composed of individual vesicles. Phragmoplast microtubules occur on both sides of the cell plate.
10 55092_3Cplate-1.jpg Instrument: JEOL TEM 1010
Microscopist: Louisa Howard

Lamiales
Lamiaceae (mint family)
Coleus Blumei

High maginfication of "2CPLate" image, showing a portion of the cell plate. At this stage of cell division, the cell plate is composed of individual vesicles. Phragmoplast microtubules occur on both sides of the cell plate.
11 Grid9c_WThypo2-3.jpg Instrument: JEOL TEM 1010
Microscopist: Louisa Howard

Brassicales
Brassicaceae (mustard family)
Arabidopsis thaliana

Transmission electron microscope image of a chloroplast in the  hypocotyl of Arabidopsis thaliana. There are starch granules present, along with the grana (stacks of disc-shaped thylakoids).
12 Grid9c_WThypo2-1.jpg Instrument: JEOL TEM 1010
Microscopist: Louisa Howard


Brassicales
Brassicaceae (mustard family)
Arabidopsis thaliana

Transmission electron microscope image of a chloroplast in the hypocotyl of Arabidopsis thaliana. There are starch granules present, along with the grana (stacks of disc-shaped thylakoids).
13 Grid9c_WThypo2-4.jpg Instrument: JEOL TEM 1010
Microscopist: Louisa Howard

Brassicales
Brassicaceae (mustard family)
Arabidopsis thaliana

Transmission electron microscope image of part of a chloroplast in the hypocotyl of Arabidopsis thaliana. There are starch granules present, along with the grana (stacks of disc-shaped thylakoids).
14 Grid9c_WThypo2-2.jpg Instrument: JEOL TEM 1010
Microscopist: Louisa Howard

Brassicales
Brassicaceae (mustard family)
Arabidopsis thaliana

Transmission electron microscope image of part of a chloroplast in the  hypocotyl of Arabidopsis thaliana. There are starch granules present, along with the grana (stacks of disc-shaped thylakoids).

Dartmouth Electron Microscope Facility