Dartmouth Electron Microscope Facility Date: Tue 07-Jun-2011 Time: 15:14
01_0395WTcholera-1.jpg Instrument: JEOL 100CX TEM
Microscopist: Louisa Howard.

Transmission electron microscope image of Vibrio choleraethat has been negatively stained. Vibrio cholerae is the bacteria responsible for the gastroinestinal disease cholera. In order to get the disease cholera, the bacteria must be able to colonize in the small intestine and a critical factor necessary for this colonization is the toxin-co-regulated pilus (TCP). 0395 is a wild type strain, showing the normal bundling of toxin-co-regulated pilus (TCP). Wild-type pili are clearly visible as 7 nm fibres that form bundles @ 0.2-0.3 µm wide and 3-6 µm long.

For more information, see the journal article: T.J. Kirn, M.J. Lafferty, C.M.P Sandoe and R.K. Taylor, 2000, "Delineation of pilin domains required for bacterial association into microcolonies and intestinal colonization", Molecular Microbiology, Vol. 35(4):896-910
02_d175a-cholera.jpg Instrument: JEOL 100CX TEM
Microscopist: Louisa Howard.

Transmission electron microscope image of Vibrio cholerae that has been negatively stained. Vibrio cholerae is the bacteria responsible for the gastroinestinal disease cholera. In order to get the disease cholera, the bacteria must be able to colonize in the small intestine and a critical factor necessary for this colonization is the toxin-co-regulated pilus (TCP). D175A is a tcpA mutant strain and the pili are clearly visible as 7 nm fibers. This mutant strain shows simlar bundling of toxin-co-regulated pilus (TCP) as that of the wild type strain(O395 WT Cholera wild type). However, this mutant strain showed a greater number of pili bundles than the wild type strain.

For more information, see the journal article: T.J. Kirn, M.J. Lafferty, C.M.P Sandoe and R.K. Taylor, 2000, "Delineation of pilin domains required for bacterial association into microcolonies and intestinal colonization", Molecular Microbiology, Vol. 35(4):896-910
03_k121a-cholera.jpg Instrument: JEOL 100CX TEM
Microscopist: Louisa Howard.

Transmission electron microscope image of Vibrio cholerae that has been negatively stained. Vibrio cholerae is the bacteria responsible for the gastroinestinal disease cholera. In order to get the disease cholera, the bacteria must be able to colonize in the small intestine and a critical factor necessary for this colonization is the toxin-co-regulated pilus (TCP). K121A is a tcpA mutant strain whose pili are clearly visible as 7 nm fibers that form bundles @ 0.2-0.3 µm wide, similar to the wild type strain(O395 WT Cholera wild type). However, when viewed at a much lower magnification than this image, the bundles in this mutant strain were much longer than the wild type strain.

For more information, see the journal article: T.J. Kirn, M.J. Lafferty, C.M.P Sandoe and R.K. Taylor, 2000, "Delineation of pilin domains required for bacterial association into microcolonies and intestinal colonization", Molecular Microbiology, Vol. 35(4):896-910
04_h181a-cholera.jpg Instrument: JEOL 100CX TEM
Microscopist: Louisa Howard.

Transmission electron microscope image of Vibrio cholerae that has been negatively stained. Vibrio cholerae is the bacteria responsible for the gastroinestinal disease cholera. In order to get the disease cholera, the bacteria must be able to colonize in the small intestine and a critical factor necessary for this colonization is the toxin-co-regulated pilus (TCP). H181A is a tcpA mutant strain. The pili are visible as 7 nm fibers. This strain had pili whose morphology was similar to the wild type.

For more information, see the journal article: T.J. Kirn, M.J. Lafferty, C.M.P Sandoe and R.K. Taylor, 2000, "Delineation of pilin domains required for bacterial association into microcolonies and intestinal colonization", Molecular Microbiology, Vol. 35(4):896-910
05_e158l-cholera-pili-loose.jpg Instrument: JEOL 100CX TEM
Microscopist: Louisa Howard.

Transmission electron microscope image of Vibrio cholerae that has been negatively stained. Vibrio cholerae is the bacteria responsible for the gastroinestinal disease cholera. In order to get the disease cholera, the bacteria must be able to colonize in the small intestine and a critical factor necessary for this colonization is the toxin-co-regulated pilus (TCP). E158L is a tcpA mutant strain. The pili are visible as 7 nm fibers. This mutant strain showed a much different appearance than the wild type strain. The pili were curled rather than straight and they didn't appear to bundle.

For more information, see the journal article: T.J. Kirn, M.J. Lafferty, C.M.P Sandoe and R.K. Taylor, 2000, "Delineation of pilin domains required for bacterial association into microcolonies and intestinal colonization", Molecular Microbiology, Vol. 35(4):896-910
06_A9RM400-1.jpg Instrument: JEOL 1010 TEM
Microscopist: Louisa Howard.

  Transmission electron microscope image of a thin section of Vibrio choleraeImage shows immunogold label of a membrane protein on Vibrio cholerae cell surface.

07_A9RM400-6.jpg Instrument: JEOL 1010 TEM
Microscopist: Louisa Howard.

Transmission electron microscope image of a thin section of Vibrio choleraeImage shows immunogold label of a membrane protein on Vibrio cholerae
08_A9RM400-4.jpg Instrument: JEOL 1010 TEM
Microscopist: Louisa Howard.

Transmission electron microscope image of a thin section of Vibrio choleraeImage shows label of a membrane protein on Vibrio cholerae
09_A9rm400-5.jpg Instrument: JEOL 1010 TEM
Microscopist: Louisa Howard.



Transmission electron microscope image of a thin section of Vibrio choleraeImage shows label of a membrane protein on Vibrio cholerae
10_A9RM400-2.jpg Instrument: JEOL 1010 TEM
Microscopist: Louisa Howard.

Transmission electron microscope image of a thin section of Vibrio choleraeImage shows immunogold label of a membrane protein on Vibrio cholerae
11_A9RM400-3.jpg Instrument: JEOL 1010 TEM
Microscopist: Louisa Howard.

Transmission electron microscope image of a thin section of Vibrio choleraeImage shows immunogold label of a membrane protein on Vibrio cholerae
12_Cholera_CJM139-2.jpg Instrument: JEOL 1010 TEM
Microscopist: Louisa Howard.

Transmission electron microscope image of Vibrio cholerae that has been negatively stained. Vibrio cholerae is the bacteria responsible for the gastroinestinal disease cholera. In order to get the disease cholera, the bacteria must be able to colonize in the small intestine and a critical factor necessary for this colonization is the toxin-co-regulated pilus (TCP).
13_Cholera_CJM139-3.jpg Instrument: JEOL 1010 TEM
Microscopist: Louisa Howard.

Transmission electron microscope image of Vibrio cholerae that has been negatively stained. Vibrio cholerae is the bacteria responsible for the gastroinestinal disease cholera. In order to get the disease cholera, the bacteria must be able to colonize in the small intestine and a critical factor necessary for this colonization is the toxin-co-regulated pilus (TCP).

Dartmouth Electron Microscope Facility