Dartmouth Electron Microscope Facility Date: Tue 13-Sep-2011 Time: 14:36
01_RootNodule80936_5KX.jpg Instrument: JEOL 1010 TEM
Microscopist: Louisa Howard.

Transmission electron microscope image of a cross section through a soybean(Glycine max.Essex) root nodule. The bacteria, Bradyrhyzobium japonicum, infects the roots and establishes a nitrogen fixing symbiosis. This image shows a cell and an infection thread. In this image, you can also see a cell in process of dividing.
For more information, see the articles:
Benson HP, Boncompagni E, Guerinot ML (2005) "An iron uptake operon required for proper nodule development in the Bradyrhizobium japonicum-soybean symbiosis" Mol Plant Microbe Interact. 2005 Sep;18(9):950-9.
Roth, E. and G. Stacey (1989) "Bacterium release into host cells of nitrogen-fixing soybean nodules"  J.C.B. Vol. 49 pp.13-23 1989
02_RootNodule80938_50KX.jpg Instrument: JEOL 1010 TEM
Microscopist: Louisa Howard.
Transmission electron microscope image of a cross section through a soybean(Glycine max.Essex) root nodule. The bacteria, Bradyrhyzobium japonicum, infects the roots and establishes a nitrogen fixing symbiosis. This high magnification image shows part of a cell with single bacteriods within their symbiosomes. In this image, you can also see endoplasmic reticulum and the cell wall.
For more information, see the articles:
Benson HP, Boncompagni E, Guerinot ML (2005) "An iron uptake operon required for proper nodule development in the Bradyrhizobium japonicum-soybean symbiosis" Mol Plant Microbe Interact. 2005 Sep;18(9):950-9.
Roth, E. and G. Stacey (1989) "Bacterium release into host cells of nitrogen-fixing soybean nodules"  J.C.B. Vol. 49 pp.13-23 1989
03_RootNodule80932_7KX.jpg Instrument: JEOL 1010 TEM
Microscopist: Louisa Howard.
Transmission electron microscope image of a cross section through a soybean(Glycine max.Essex) root nodule. The bacteria, Bradyrhyzobium japonicum, infects the roots and establishes a nitrogen fixing symbiosis. This image shows a cell with single bacteriods within their symbiosomes.
For more information, see the articles:
Benson HP, Boncompagni E, Guerinot ML (2005) "An iron uptake operon required for proper nodule development in the Bradyrhizobium japonicum-soybean symbiosis" Mol Plant Microbe Interact. 2005 Sep;18(9):950-9.
Roth, E. and G. Stacey (1989) "Bacterium release into host cells of nitrogen-fixing soybean nodules"  J.C.B. Vol. 49 pp.13-23 1989
04_RootNodule80937_33KX.jpg Instrument: JEOL 1010 TEM
Microscopist: Louisa Howard.

Transmission electron microscope image of a cross section through a soybean(Glycine max.Essex) root nodule. The bacteria, Bradyrhyzobium japonicum, infects the roots and establishes a nitrogen fixing symbiosis. This image shows a cell with single bacteriods within their symbiosomes.
For more information, see the articles:
Benson HP, Boncompagni E, Guerinot ML (2005) "An iron uptake operon required for proper nodule development in the Bradyrhizobium japonicum-soybean symbiosis" Mol Plant Microbe Interact. 2005 Sep;18(9):950-9.
Roth, E. and G. Stacey (1989) "Bacterium release into host cells of nitrogen-fixing soybean nodules"  J.C.B. Vol. 49 pp.13-23 1989
05_RootNodule80928_3KX.jpg Instrument: JEOL 1010 TEM
Microscopist: Louisa Howard.

Transmission electron microscope image of a cross section through a soybean(Glycine max.Essex) root nodule. The bacteria, Bradyrhyzobium japonicum, infects the roots and establishes a nitrogen fixing symbiosis. This image shows cells, with bacteriods within their symbiosomes, and infection threads
For more information, see the articles:
Benson HP, Boncompagni E, Guerinot ML (2005) "An iron uptake operon required for proper nodule development in the Bradyrhizobium japonicum-soybean symbiosis" Mol Plant Microbe Interact. 2005 Sep;18(9):950-9.
Roth, E. and G. Stacey (1989) "Bacterium release into host cells of nitrogen-fixing soybean nodules"  J.C.B. Vol. 49 pp.13-23 1989
06_Grid13b_Soy_Rhyzobia-1.jpg Instrument: JEOL 1010 TEM
Microscopist: Louisa Howard.

Transmission electron microscope image of a cross section through a soybean(Glycine max.Essex) root nodule. The bacteria, Bradyrhyzobium japonicum, infects the roots and establishes a nitrogen fixing symbiosis. This  image shows cells with bacteriods within their symbiosomes and infection threads.

For more information, see the articles:
Benson HP, Boncompagni E, Guerinot ML (2005) "An iron uptake operon required for proper nodule development in the Bradyrhizobium japonicum-soybean symbiosis" Mol Plant Microbe Interact. 2005 Sep;18(9):950-9.
Roth, E. and G. Stacey (1989) "Bacterium release into host cells of nitrogen-fixing soybean nodules"  J.C.B. Vol. 49 pp.13-23 1989
07_SoyB_Rhyz_1sectored-3.jpg Instrument:  FEI XL-30 FEG ESEM
Microscopist: Louisa Howard.

Scanning electron microscope image of a cross section though a soybean(Glycine max.Essex) root nodule. The bacteria, Bradyrhyzobium japonicum, infects the roots and establishes a nitrogen fixing symbiosis.

For more information, see the articles:
Benson HP, Boncompagni E, Guerinot ML (2005) "An iron uptake operon required for proper nodule development in the Bradyrhizobium japonicum-soybean symbiosis" Mol Plant Microbe Interact. 2005 Sep;18(9):950-9.
Roth, E. and G. Stacey (1989) "Bacterium release into host cells of nitrogen-fixing soybean nodules"  J.C.B. Vol. 49 pp.13-23 1989
08_SoyB_Rhyz_P4929_Oct19_06-3.jpg Instrument: FEI XL-30 FEG ESEM
Microscopist: Louisa Howard.

Scanning electron microscope image of a cross section though a soybean(Glycine max.Essex) root nodule. The bacteria, Bradyrhyzobium japonicum, infects the roots and establishes a nitrogen fixing symbiosis.

For more information, see the articles:
Benson HP, Boncompagni E, Guerinot ML (2005) "An iron uptake operon required for proper nodule development in the Bradyrhizobium japonicum-soybean symbiosis" Mol Plant Microbe Interact. 2005 Sep;18(9):950-9.
Roth, E. and G. Stacey (1989) "Bacterium release into host cells of nitrogen-fixing soybean nodules"  J.C.B. Vol. 49 pp.13-23 1989
09_SoyB_Rhyz_P4929_Oct19_06-4.jpg Instrument: FEI XL-30 FEG ESEM
Microscopist: Louisa Howard.

Scanning electron microscope image of a cross section though a soybean(Glycine max.Essex) root nodule. The bacteria, Bradyrhyzobium japonicum, infects the roots and establishes a nitrogen fixing symbiosis.

For more information, see the articles:
Benson HP, Boncompagni E, Guerinot ML (2005) "An iron uptake operon required for proper nodule development in the Bradyrhizobium japonicum-soybean symbiosis" Mol Plant Microbe Interact. 2005 Sep;18(9):950-9.
Roth, E. and G. Stacey (1989) "Bacterium release into host cells of nitrogen-fixing soybean nodules"  J.C.B. Vol. 49 pp.13-23 1989
10_SoyB_Rhyz_ABminus_Oct19_06-4.jpg Instrument: FEI XL-30 FEG ESEM
Microscopist: Louisa Howard.

Scanning electron microscope image of a cross section though a soybean(Glycine max.Essex) root nodule. The bacteria, Bradyrhyzobium japonicum, infects the roots and establishes a nitrogen fixing symbiosis.

For more information, see the articles:
Benson HP, Boncompagni E, Guerinot ML (2005) "An iron uptake operon required for proper nodule development in the Bradyrhizobium japonicum-soybean symbiosis" Mol Plant Microbe Interact. 2005 Sep;18(9):950-9.
Roth, E. and G. Stacey (1989) "Bacterium release into host cells of nitrogen-fixing soybean nodules"  J.C.B. Vol. 49 pp.13-23 1989
11_SoyB_Rhyz_ABminus_Oct19_06-8.jpg Instrument: FEI XL-30 FEG ESEM
Microscopist: Louisa Howard.

Scanning electron microscope image of a cross section though a soybean(Glycine max.Essex) root nodule. The bacteria, Bradyrhyzobium japonicum, infects the roots and establishes a nitrogen fixing symbiosis.

For more information, see the articles:
Benson HP, Boncompagni E, Guerinot ML (2005) "An iron uptake operon required for proper nodule development in the Bradyrhizobium japonicum-soybean symbiosis" Mol Plant Microbe Interact. 2005 Sep;18(9):950-9.
Roth, E. and G. Stacey (1989) "Bacterium release into host cells of nitrogen-fixing soybean nodules"  J.C.B. Vol. 49 pp.13-23 1989

Dartmouth Electron Microscope Facility