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E158L Cholera tcpA mutant
Transmission electron microsope image of Vibrio choleraethat has been negatively stained. Vibrio choleraeis the bacteria responsible for the gastroinestinal disease cholera. In order to get the disease cholera, the bacteria must be able to colonize in the small intestine and a critical factor necessary for this colonization is the toxin-co-regulated pilus(TCP). E158L is a tcpA mutant strain. The pili are visible as 7 nm fibres. This mutant strain showed a much different appearance than the wild type strain. The pili were curled rather than straight and they didn't appear to bundle.
For more information, see the journal article: T.J. Kirn, M.J. Lafferty, C.M.P Sandoe and R.K. Taylor, 2000, "Delineation of pilin domains required for bacterial association into microcolonies and intestinal colonization", Molecular Microbiology, Vol. 35(4):896-910
To go to the article, click here.


Bacteria, TEM, Vibrio cholerae, pili, tcp
Tom Kirn, Ron Taylor, Louisa Howard